Entomological study on vectorial density, temporal variation of tsetse fly and other biting flies in intervention and non-intervention areas of South Omo Zone, Ethiopia.

A repeated cross-sectional entomological survey was conducted to estimate Glossina (tsetse) and other biting flies density, their seasonal variation and associated risk factors in intervention and non- intervention areas of South Omo Zone, Southwest Ethiopia from January 2019-November 2019. In both dry and wet seasons, a total of 96 NGU traps (64 traps in tsetse intervention districts and 32 traps in tsetse non- intervention districts) were deployed at an interval of about 100-200 m in purposively selected and suspected tsetse habitats. Thus, Glossina pallidipes was found to be the only cyclical vector along with mechanical vectors of Tabanus, Stomoxys and Haematopota. In tsetse intervention areas, G. pallidipes apparent density of 2.64 F/T/D and 0.42 F/T/D was recorded in dry and wet season respectively. Mechanical vectors (dry; wet) of Tabanus (205; 155), Stomoxys (34; 54) and Haematopota (50; 33) were also recorded in tsetse intervened areas. Whereas, in non- intervention areas, apparent density of G. pallidipes was 2.03 F/T/D and 0.56 F/T/D, respectively in dry and wet season. Similarly, Tabanus (22; 56), Stomoxys (10; 8) and Haematopota (5; 7) respectively in dry and wet (dry; wet) season were recorded in tsetse non- intervention areas. According to Negative Binomial Regression (NBR), season was the only variable significantly affecting (P < 0.05) the Glossina count in the current study area. Accordingly, the incidence G. pallidipes during wet season was decreased by the factor of 0.21 (CI; 0.097-0.47) when compared to its incidence in dry season by holding other variables constant. In conclusion, cyclical vectors were playing vital role in transmission of trypanosomosis in South Omo Zone along with numerous mechanical vectors even though there have been vector intervention activities in the areas. Therefore, strong, sustainable, environmentally friend and community participating vector control strategies should be followed to tackle the vector distribution in the area.

Knowledge, attitude and practices study of acaricide usage and tick control in South Omo Zone pastoral areas, South-Western Ethiopia.

Although acaricide chemotherapy is widely used to control tick infestation in Ethiopia, its effectiveness is uncertain due to misusage by herdsmen. Currently, there is no study being conducted in the South Omo Zone of Ethiopia which shows the knowledge, attitude, and practice (KAP) and associated factors of acaricide usage by herdsmen. Therefore, this study was conducted to assess KAP of 120 (83 male and 37 female) pastoralist and agro-pastoralist of Bena-Tsemay district through structured questionnaire survey. Accordingly, Ivermectin was the most preferred acaricide by majority (62.5%) of the herdsmen. Half (50%) of the herdsmen confessed that price of acaricide is the defining variable for acaricide preference in their location where 60.83% of them obtain acaricides from private drug shops. Majority (60%) of the respondents said that they obtain information about acaricide usage from drug sellers in the vet drug shops. According to 72.50% of the respondents, acaricide application/injection on the infested herd was conducted by the herdsmen. A 95.83% of our interviewee revealed that there was no training or awareness creation being given on how to inject or apply acaricide on tick infested animals. Moreover, all responders (100%) confessed that they didn't have a practice of weighing animals and measuring acaricide dosage prior to injection/application. The incidence of acaricide poisoning on animal and personnel was reported by 19.17% and 22.5% of respondents, respectively. Simple logistic regression analysis revealed that gender (OR = 5.09, OR 95% CI = 2.30-11.72), practice of acaricide rotation (OR = 3.22, OR 95% CI = 1.41-7.64) and personnel preference for acaricide application (OR = 2.66, OR 95% CI = 1.18-6.15) were significantly (P < 0.05) associated with the knowledge score of the respondents. On the other hand, respondent's attitude score was significantly (P < 0.05) associated with their acaricide rotation practice (OR = 3.20, OR 95% CI = 1.39-7.53) and personnel preference for acaricide application (OR = 6.61, OR 95% CI = 2.78-16.93). Similarly, practice of acaricide rotation (OR = 5.31, OR 95% CI = 2.26-12.96) and personnel preference for acaricide application (OR = 7.21, OR 95% CI = 3.03-17.99) were significantly linked with the practice score of the respondents towards acaricide usage. In conclusion, ticks are the major challenge in the study area despite widespread usage of acaricides. Because of extensive misusage of available acaricides, awareness creation should be applied to narrow KAP gaps and to conserve the efficacy of these chemicals. Furthermore, acaricide efficacy investigation (in vitro and in vivo) should be conducted to know the status of commonly used acaricides in the area.

Prevalence, species composition, and associated risk factors of small ruminant gastrointestinal nematodes in South Omo zone, South-western Ethiopia.

OBJECTIVE: This study was conducted to generate data on the prevalence, species composition, and factors associated with small ruminant nematode infection in the South Omo zone, in South-western Ethiopia. MATERIAL AND METHODS: A cross-sectional study through fecal nematode parasite examination was conducted. Simple floatation test and coproculture, followed by Baermann technique, were used for nematode egg and third-stage larvae (L3) separation and identification. The McMaster method was used to calculate the egg per gram of feces (EPG). RESULTS: From a total of 242 sheep and goats examined, 72.34% were infested with single or mixed nematode parasites. District, "Kebele", species, body condition score, and age were significantly (p < 0.05) associated with nematode infestation. Simple logistic regression analysis indicated that nematode infestation in Bena-Tsemay district (78.33%) was significantly (p < 0.05) higher by a factor of 0.54 (OR 95% CI: 0.30-0.96) than Hamer district (66.39%). Among the species, caprine (79.43%) was significantly (p < 0.05) infested than ovine (62.37%) by a factor of 0.45 (OR 95% CI: 0.25-0.81). Moreover, infestation on poor (79.12%) and medium (70.96%) body conditioned animals was higher by a factor of 2.94 (OR 95% CI: 1.41-6.26) and 1.76 (OR 95% CI: 0.88-3.53) than on good body conditioned animals (63.79%). Likewise, infestation in age groups of 1-3 years (78.66%) and >3 years (84.40%) was significantly (p < 0.05) higher by a factor of 4.83 (OR 95% CI: 2.31-10.46) and 8.23 (OR 95% CI: 3.98-17.75) than younger age groups (41.37%), respectively. A moderate parasitic burden was observed on 52.90% of gastrointestinal nematodes (GIN)-infested animals with significantly (p < 0.05) higher EPG in females than males. Furthermore, mixed infestation of Trichostrongylus axei and Eimeria (6.19%), Haemonchus contortus and Eimeria (5.78%), and Trichostrongylus vitrines and Eimeria (5.78%) were dominantly identified. On the contrary, T. axei (15.70%), Eimeria (8.67%), H. contortus (7.43%), and Trichostrongylus colubriformis (7.02%) were dominant single infestations. CONCLUSION: The current study revealed the highest prevalence of GIN in the study area, which needs strategic control, needs to enhance community awareness toward GIN control and prevention, and to implement further investigation into anthelminthic efficacy to solve the problem.